Hyperpolarized 13C MR spectroscopic imaging can be used to monitor Everolimus treatment in vivo in an orthotopic rodent model of glioblastoma

Article ID	Journal	Published Year	Pages	File Type
6033066	NeuroImage	2012	9 Pages	PDF

Abstract

âº We use hyperpolarized 13C MRSI to monitor mTOR-targeted therapy in glioblastoma. âº Hyperpolarized lactate-to-pyruvate ratio drops early following onset of treatment. âº Tumor volume remains unchanged until late time points during treatment. âº The drop in lactate-to-pyruvate ratio is associated with a drop in LDH levels. âº Lactate-to-pyruvate ratio is an early biomarker of drug target modulation.

Keywords

CASP3 HIF-1α GBM DNP MRS FOV hyperpolarization mTOR inhibition mTOR FSE FVIII NEX Hyperpolarized MRSI PI3K FDG-PET CA-IX H&E Spin-echo MRI Everolimus Immunohistochemistry IHC Magnetic resonance spectroscopic imaging bioluminescence imaging Magnetic resonance imaging number of excitations computerized axial tomography Intracranial intraperitoneal BLI blood brain barrier BBB Fast spin-echo magnetic resonance spectroscopy hypoxia-inducible factor 1α Factor VIII phosphatidylinositol-3-kinase Dynamic nuclear polarization lactate dehydrogenase LDH Field of view NADH NAD, nicotinamide adenine dinucleotide mammalian target of rapamycin Hematoxylin and Eosin caspase 3 Carbonic anhydrase IX Glioblastoma