Optical probing of sodium dynamics in neurons and astrocytes

Changes in intracellular Na+ concentration underlie essential neurobiological processes, but few reliable tools exist for their measurement. Here we characterize a new synthetic Na+-sensitive fluorescent dye, Asante Natrium Green (ANG), with unique properties. This indicator was excitable in the visible spectrum and by two-photon illumination, suffered little photobleaching and located to the cytosol were it remained for long durations without noticeable unwanted effects on basic cell properties. When used in brain tissue, ANG yielded a bright fluorescent signal during physiological Na+ responses both in neurons and astrocytes. Synchronous electrophysiological and fluorometric recordings showed that ANG produced accurate Na+ measurement in situ. This new Na+ indicator opens innovative ways of probing neuronal circuits.

► Asante Natrium Green is a novel sodium-sensitive fluorescent dye. ► It can be excited with visible light and two-photon illumination. ► It gives bright stable signal with no cytotoxicity in brain tissue. ► Neuronal and astrocytic responses are detected with unmatched performance. ► This dye is suitable for studies in brain slices and in vivo.