Molecular weight determination of hydrolyzed collagens

This paper relates the comparison of three methods applied to characterize the molecular weight of hydrolyzed collagen (HC). The high-pressure size-exclusion chromatography (HPSEC) performed on hydrophilic macroporous copolymer beads with an aqueous denaturing eluent allows the determination of the molecular weights distribution and of the weight—and number-average molecular weights. The viscosity measured in standard conditions and the number-average molecular weight determined by formol titration are correlated respectively with the weight-average molecular weight (log scale) and the number-average molecular weight determined by HPSEC.