Polyenephosphatidylcholine prevents alcoholic liver disease in PPARÎ±-null mice through attenuation of increases in oxidative stress

Article ID	Journal	Published Year	Pages	File Type
6110191	Journal of Hepatology	2009	11 Pages	PDF

Abstract

PPC exhibited anti-inflammatory, anti-apoptotic, and anti-fibrotic effects on ALD as a result of inhibition of the overexpression of ROS-generating enzymes. Our results demonstrate detailed molecular mechanisms of the anti-oxidant action of PPC.

Keywords

CYP2E1 IL-1β MCP-1 IκB iNOS MPT AP-1 TLR-4 ICAM-1 COX-2 AOX GAPDH ASK1 GPX ADH αSMA COL1A1 MYD88 acyl-CoA oxidase PPC 4-hydroxynonenal 4-HNE Nox-4 MDA PI3K NF-κB ALDH MAPK NOx α smooth muscle actin aldehyde dehydrogenase acyl-coenzyme A oxidase sodium dodecyl sulfate-polyacrylamide gel electrophoresis SDS-PAGE Alcohol dehydrogenase mitochondrial permeability transition standard deviation Interleukin-1β terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling tumor necrosis factor-α TUNEL SOD inducible nitric oxide synthase Superoxide dismutase cytochrome P450 2E1 cyclo-oxygenase 2 myeloid differentiation factor 88 TNF-α nuclear factor-κB phosphatidylinositol-3 kinase malondialdehyde inhibitor of NF-κB intercellular adhesion molecule-1 Monocyte chemotactic protein-1 activator protein-1 mitogen-activated protein kinase apoptosis signal-regulating kinase 1 glutathione peroxidase glyceraldehyde-3-phosphate dehydrogenase