| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6113991 | Transfusion and Apheresis Science | 2015 | 4 Pages |
Abstract
ECP should be validated: we compared in parallel apoptosis and proliferation analysis of patient lymphocytes treated with 8-MOP ECP using respectively Annexin V/7-aminoactinomycin D (7-AAD) and CFSE with a tetrazolium salt (WST-1) method. Using WST-1 assay we found a significant decrement (pâ<â0.01) of metabolic activity at 4 days between ECP-treated and untreated cells. This finding was confirmed by the significant decrease of cell proliferation and increase of cell death observed by CFSE and 7AAD-Annexin V, respectively. Accordingly, once validated against a reference method, WST-1 could represent a rapid and easy assay for routinely quality control of ECP.
Related Topics
Health Sciences
Medicine and Dentistry
Hematology
Authors
Katia Chieregato, Alberta Alghisi, Carlo Borghero, Francesca Elice, Roberto Raimondi, Ermella Zanetti, Francesco Rodeghiero, Giuseppe Astori,