| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6115421 | Diagnostic Microbiology and Infectious Disease | 2016 | 5 Pages |
Abstract
Analytically accurate human papillomavirus (HPV) genotyping methods are required to assess the impact of HPV vaccination. The aim of this study was to evaluate the analytical performance of Anyplex II HPV28 (Seegene, Korea) and Euroarray HPV (Euroimmun, Germany) genotyping kits, for conducting a future HPV vaccine efficacy monitoring study in Luxembourg. A total number of 150 cervical swabs were collected from women with mean age 31.4 years. Agreements for detecting any HPV between Aptima/Anyplex (88.0%) and Aptima/Euroarray (90.7%) were similar. Agreement of Anyplex/EuroArray with Aptima was higher for Genotypes 16, 18 or 45 than for the other 11 HPVs. The average number of HPV genotypes detected per sample was similar with 2.6 and 2.5, for Anyplex and EuroArray, respectively. In conclusion, Anyplex and Euroarray showed high agreement in general and in particular for detecting genotypes contained in HPV vaccines.
Keywords
mRNAICCRT-PCRHPV Genotypingmessenger RNAInternational Agency for Research on CancerIARC یا International Agency for Research on Cancerinvasive cervical cancerconfidence intervalgenome equivalentsMethod comparisoninternational unitspolymerase chain reactionreal-time polymerase chain reactionPCRHuman papillomavirusHuman papilloma virusHPV
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Authors
Ardashel Latsuzbaia, Jessica Tapp, Trung Nguyen, Marc Fischer, Marc Arbyn, Steven Weyers, Joël Mossong,