Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6115627 | Diagnostic Microbiology and Infectious Disease | 2016 | 5 Pages |
Abstract
Our study was aimed to identify the phenotypic and genotypic pyrazinamide (PZA) resistance features among multidrug-resistant (MDR) isolates in a national tuberculosis (TB) referral center of China. PZA susceptibility test was performed for a total of 142 MDR-TB clinical isolates using the MGIT 960 PZA kits, and the pncA, rpsA, and panD genes were sequenced. Extensively drug-resistant (XDR) and pre-XDR strains had higher PZA resistance rate than that of MDR strains which were still sensitive to fluoroquinolone and aminoglycoside (42.9%, 24/56) (Ï2Â =Â 8.922, PÂ =Â 0.012). No panD mutation was detected among the PZA resistant strains with wild-type pncA and rpsA genes. Our study indicates that PZA-resistant frequency increases with TB drug resistance level; pncA, rpsA, and panD mutations had strong, low, and no correlation with PZA resistance, and rapid molecular assay will facilitate the timely identification of the PZA-sensitive MDR-TB.
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Authors
Yunting Gu, Xia Yu, Guanglu Jiang, Xiaobo Wang, Yifeng Ma, Yunxu Li, Hairong Huang,