Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6115646 | Diagnostic Microbiology and Infectious Disease | 2016 | 5 Pages |
Abstract
We compared in an vitro model the yields of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and conventional culture (CC) for the detection of catheter colonization with superficial catheter samples (SS). We used blood culture bottles (BCB) with an inserted cannula and incubated at 37 °C. The BCB were manipulated with different contaminations and when a BCB turned positive, SS were obtained to perform both techniques. To compare both techniques we analyzed the mean time to colonization (MTC) and the mean time to a result (MTR). The MTC (SD, days) by CC and MALDI-TOF was as follows: hub, 0.59 (0.79) versus 1.07 (1.39), P = 0.06; surface: 0.62 (0.67) versus 0.82 (0.81), P < 0.001. The MTR (SD, days) of CC and MALDI-TOF was as follows: hub: 1.58 (0.79) versus 2.25 (1.48), P = 0.04; surface: 1.62 (0.67) versus 1.95 (0.80), P < 0.001. In general, the use of MALDI-TOF performed directly with SS was no better than CC and did not anticipate colonization results.
Related Topics
Life Sciences
Immunology and Microbiology
Applied Microbiology and Biotechnology
Authors
MarÃa Guembe, MarÃa Jesús Pérez-Granda, Raquel Cruces, Belén RodrÃguez-Sánchez, Adrián RuÃz, Luis Alcalá, Emilio Bouza,