Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6116238 | Diagnostic Microbiology and Infectious Disease | 2013 | 5 Pages |
Abstract
Carbohydrates of pathogen antigens have been disrupted by periodate oxidation, in order to reduce nonspecific bindings and improve serodiagnosis of parasite infections. In the present study, the enzyme-linked immunosorbent assay (ELISA) was carried out with filariform larvae antigen treated, or not treated, with sodium metaperiodate. Groups of sera from patients with Strongyloides stercoralis infection, with other intestinal parasites and a normal control, were used. The oxidation of Strongyloides stercoralis glycosylated epitopes reduced the seroreactivity of sera from patients with S. stercoralis infection as demonstrated by ELISA, with a decrease in sera optical densities. The number of cross-reactions of IgG and IgE-ELISAs increased by 12% and 16%, respectively, after antigen treatment with metaperiodate. This was more often observed in patients infected with Schistosoma mansoni and hookworm. Moreover, the IgG depletion from sera tested by IgE-ELISA led to the detection of previous false-negative samples from S. stercoralis-infected patients.
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Authors
Elizabete de Jesus Inês, Mônica Lopes Sampaio Silva, Joelma Nascimento Souza, Márcia Cristina Aquino Teixeira, Neci Matos Soares,