Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6116292 | Diagnostic Microbiology and Infectious Disease | 2011 | 7 Pages |
Abstract
Matrix-assisted laser desorption-ionization time of flight mass spectrometry (MALDI-TOF MS) combined with weak cationic exchange (WCX) magnetic beads was used to establish a decision tree model that distinguished extrapulmonary tuberculosis (EPTB) from non-EPTB individuals. Eight-one patients with EPTB and 112 non-EPTB individuals (72 disease controls and 40 healthy controls) were involved in this study. The model was set up by 5 of 19 differentially expressed peaks (P < 0.05), m/z 4100, 4310, 6093, 8605, and 14,019. This model can discriminate patients with EPTB from non-EPTB with a sensitivity of 97.7% and a specificity of 84.1%. The test set verified that this model had good sensitivity and specificity: 94.4% and 83.6%, respectively. In conclusion, MALDI-TOF MS combined with WCX magnetic beads is a powerful technology for constructing a decision tree model and the model we built could serve as a potential diagnostic tool for EPTB.
Related Topics
Life Sciences
Immunology and Microbiology
Applied Microbiology and Biotechnology
Authors
Chuiwen Deng, Minggui Lin, Chaojun Hu, Yanfeng Li, Yang Gao, Xiaoxing Cheng, Fengchun Zhang, Mei Dong, Yongzhe Li,