Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6116784 | Human Immunology | 2013 | 5 Pages |
Abstract
The Major Histocompatibility Complex Class I Chain-Related Gene A (MICA) is located 46.4Â Kb centromeric to HLA-B locus on chromosome 6; 84 alleles have been described so far. To assess the distribution of MICA alleles in an Austrian population, 322 unrelated Austrian blood donors have been typed for MICA by direct sequencing of amplified exons 2-5; sequencing of exon 6 and separating alleles by haplotype specific primers or by cloning was performed to resolve ambiguities. HLA-B was typed at low level resolution and linkage disequilibrium was determined. We observed 20 already known and four novel MICA alleles. MICAâ008:01/04 was the most frequent allele (42%), followed by MICAâ002:01 (11%) and MICAâ009:01 (9%), three alleles (MICAâ029, â067 and â068) were observed only once. No deviation from the Hardy Weinberg equilibrium was observed. Linkage disequilibrium between MICA and HLA-B alleles was observed, most extensively between MICAâ008:01/04 and HLA-Bâ07. Our population data are in agreement with other European populations. The fact that four novel alleles have been observed indicates that the polymorphism of MICA is larger than currently estimated.
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Authors
Sabine Wenda, Ingrid Faé, Alicia Sanchez-Mazas, José M. Nunes, Wolfgang R. Mayr, Gottfried F. Fischer,