Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6117608 | International Journal of Antimicrobial Agents | 2016 | 5 Pages |
Abstract
Considering that most patients who experience virological failure (VF) on lopinavir-based antiretroviral therapy (ART) fail due to poor adherence rather than resistance, an objective adherence measure could limit costs by rationalising the use of genotype antiretroviral resistance testing (GART) in countries with access to third-line ART. A cross-sectional study was conducted in a resource-limited setting at two large clinics in Kwazulu-Natal, South Africa, in patients experiencing VF (HIV-RNAâ>â1000 copies/mL) on lopinavir-based ART who had undergone GART. Associations between major protease inhibitor (PI) resistance mutations and random plasma lopinavir concentrations were explored. A total of 134 patients, including 31 children, were included in the analysis. The prevalence of patients with major PI resistance mutations was 20.9% (nâ=â28). A random lopinavir concentration above the recommended minimum trough of 1âµg/mL [adjusted odds ratio (aOR)â=â5.81, 95% confidence interval (CI) 2.04-16.50; Pâ=â0.001] and male sex (aORâ=â3.19, 95% CI 1.22-8.33; Pâ=â0.018) were predictive of the presence of at least one major PI resistance mutation. Random lopinavir concentrations of <1âµg/mL had a negative predictive value of 91% for major PI resistance mutations. Random lopinavir concentrations are strongly associated with the presence of major PI resistance mutations. Access to costly GART in patients experiencing VF on second-line ART could be restricted to patients with lopinavir concentrations above the recommended minimum trough of 1âµg/mL or, in areas where GART is unavailable, could be used as a criterion to empirically switch to third-line ART.
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Authors
Richard Court, Michelle Gordon, Karen Cohen, Annemie Stewart, Bernadett Gosnell, Lubbe Wiesner, Gary Maartens,