Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6127920 | Acta Tropica | 2013 | 7 Pages |
Abstract
Forty-two Bacillus sphaericus strains were isolated from soil around Tamil Nadu, India. Phylogenetic relationship and presence of mosquito larvicidal genes in the isolates were analysed by REP-PCR and multiplex PCR respectively. Three isolates showed high level of toxicity and they were grouped distinctly from other B. sphaericus isolates by dendrogram generated from REP-PCR. The mosquito larvicidal activity was mainly attributed by BinA and BinB were observed. Among Mtx toxins, Mtx1 plays a vital role in toxicity was elucidated. Both REP-PCR and multiplex PCR could be developed in screening of novel highly toxic B. sphaericus strains from environment without bioassay.
Related Topics
Life Sciences
Immunology and Microbiology
Parasitology
Authors
D. Immanual Gilwax Prabhu, S. Gowri Sankar, P. Thirumalai Vasan, P. Sobana Piriya, B. Karpanai Selvan, S. John Vennison,