A vaccine formulation consisting of nucleocapsid-like particles from Dengue-2 and the fusion protein P64k-domain III from Dengue-1 induces a protective immune response against the homologous serotypes in mice

► We evaluate in mice a vaccine against Dengue-1 and 2 employing alum as adjuvant. ► The formulation induces neutralizing antibodies only against Dengue-1. ► The spleen cells secrete IFN gamma after the stimulation with both serotypes. ► High levels of protection were achieved after the intracranial challenge.