| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6128070 | Acta Tropica | 2012 | 7 Pages |
Abstract
⺠We developed a fluorescence microscopy assay for detecting environmental leptospires. ⺠Leptospires were well visible using single and multiple serovar assay variants. ⺠Assay detection limits in real water samples ranged between 101 and 102 cells per mL. ⺠Applicability of the single serovar assay was shown in a pilot field study.
Keywords
IMSDAOSMIMSEMJHDFAEPAFITCPBSBSAbovine serum albuminEnvironmental Protection AgencyAcridine orangeCapture efficiencyMicroscopic Agglutination TestAntigen detectionImmuno-magnetic separationRoom temperatureDiagnostic methodsPhosphate buffered salinefluorescein isothiocyanateLeptospirosisMatFluorescence microscopy
Related Topics
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Immunology and Microbiology
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Authors
Stefan Schreier, Galayanee Doungchawee, Darapond Triampo, Piyada Wangroongsarb, Rudi A. Hartskeerl, Wannapong Triampo,