Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6132789 | Journal of Virological Methods | 2016 | 4 Pages |
Abstract
Assay artifacts were reported in plate-based immuoassays during the assessment of specific molecular interactions owing to the surface induced aggregation/conformational changes. To circumvent surface adsorption and associated artifacts, we used a solution competition ELISA by allowing unrestricted interaction between binding partners to occur in solution for better discrimination between epitopes with subtle differences. A difference of two orders of magnitude in binding to neutralizing antibodies for two truncated versions of the hepatitis E virus capsid protein was observed, while other assays showed comparable antigenicity with the same monoclonal antibodies. Discrimination of epitopes with high degree resemblance in analogous viral capsid proteins was demonstrated quantitatively based on their specific interactions. Therefore, the solution competition ELISA is a method of choice when the detection of subtle differences of two highly analogous proteins is desired.
Keywords
Related Topics
Life Sciences
Immunology and Microbiology
Virology
Authors
Lu Cao, Xin Wang, Mujin Fang, Ningshao Xia, Qinjian Zhao,