| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6132801 | Journal of Virological Methods | 2016 | 6 Pages |
Abstract
Precise calculation of DNA loads in samples containing a wide range of viral amounts was allowed by generating a standard curve for absolute quantification. The assay was proven to be highly specific, since no cross-reactions with the different CAdV type was observed, and sensitive, being able to detect less than 10 copies of CAdV-1/CAdV-2 DNA. The low intra-assay and interassay coefficient of variations demonstrated a high repeatability, thus confirming the potential use of this assay for quantitative detection of CAdV-1 and CAdV-2 for rapid diagnosis and epidemiological investigations.
Keywords
Related Topics
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Immunology and Microbiology
Virology
Authors
Giulia Dowgier, Viviana Mari, Michele Losurdo, Vittorio Larocca, Maria Loredana Colaianni, Francesco Cirone, Maria Stella Lucente, Vito Martella, Canio Buonavoglia, Nicola Decaro,