Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6132922 | Journal of Virological Methods | 2016 | 13 Pages |
Abstract
The results show that N-terminally biotinylated peptides can bind to avidin conjugated beads, and function in detection of the corresponding monoclonal antibody WH303. There are indications that the length of the spacer between epitope and biotin affect the efficiency of the peptide-antibody interaction. A protocol was established that enables probing for antibodies in porcine sera, where neutravidin-blocking of serum and the use of empty control beads for normalization was crucial. With a set of porcine sera with antibodies against various pestiviruses, the proof of concept of a peptide-based suspension array for specific detection of antibodies against pestiviruses in porcine sera was demonstrated.
Keywords
Related Topics
Life Sciences
Immunology and Microbiology
Virology
Authors
Fimme J. van der Wal, Tinka Jelsma, Helmi Fijten, René P. Achterberg, Willie L.A. Loeffen,