Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6132981 | Journal of Virological Methods | 2015 | 8 Pages |
Abstract
An all-in-one (AIO) dry-reagent time-resolved fluorometric immunoassay that requires minimal liquid handling was developed for the detection of anti-HIV-1 and -2 antibodies. To prepare the AIO wells, in vivo biotinylated capture antigens (r-Bio-HIV-1env and r-Bio-HIV-2env) were immobilized on streptavidin-coated microtitration wells and Eu(III) chelate labelled non-biotinylated tracer antigens [r-HIV-1env-Eu(III) and r-HIV-2env-Eu(III)] were dried in stable form in the same wells. The HIV AIO assay was evaluated with serum/plasma samples (n = 148) from in-house and commercial panels at two different incubation times of 15 min and 1 h. The overall sensitivity of the AIO assay was 98.6% and specificity was 100% for both the incubation times. The AIO assay can accept whole blood matrix. This assay is envisioned to fill the gap between the rapid point-of-care assays and traditional enzyme immunoassays (EIA) in terms of complexity and turnaround time, without compromising the performance.
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Authors
Sheikh M. Talha, Etvi Juntunen, Teppo Salminen, Amninder Sangha, Tytti Vuorinen, Navin Khanna, Kim Pettersson,