| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6133045 | Journal of Virological Methods | 2015 | 6 Pages |
Abstract
The results showed that the expressed products protein E0 in the L. acidophilus LA-5 resulted in single band of 27 kDa by SDS-PAGE and its strong reactivity with BVDV antibody was confirmed by Western blotting. The IgG and sIgA antibodies responses, IL-12 and IFN-γ expression levels in the vaccinated mice with recombinant L. acidophilus pMG36e-E0-LA-5 were significantly higher than those in the control mice. The protective rate of the vaccinated mice against BVDV increased significantly, and a 90.00% protection rate in virulent challenge was observed. These results indicated that the recombinant L. acidophilus pMG36e-E0-LA-5 strain was successfully constructed and it could effectively improve the immune response in mice and might provide protection against BVDV.
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Authors
Yuelan Zhao, Lufeng Jiang, Teng Liu, Min Wang, Wenbo Cao, Yongzhan Bao, Jianhua Qin,