Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6133088 | Journal of Virological Methods | 2015 | 5 Pages |
Abstract
Viral hemorrhagic septicemia virus (VHSV) has been reported to be stable in both fresh as well as seawater, suggesting that VHSV exists in natural aquatic environments and might have an effect on the wild and cultured fish. However, VHSV is below the detectable limits of laboratory tests in natural seawater. In this study, a two-step ultrafiltration (UF) procedure was used to concentration of VHSV in seawater, providing samples that were tested for infectivity by cell culture and the presence of VHSV by quantitative reverse transcriptase PCR (qRT-PCR) methods. Overall, VHSV was approximately concentrated 100-1000 times in 1, 5 and 10 L, seawater volumes respectively: from 2.81Â ÃÂ 106 to 6.53Â ÃÂ 107/mL and 103.3 to 103.8Â TCID50/mL prior to the UF procedure, to 3.78Â ÃÂ 108, 1.16 Ã 1011, and 9.12 Ã 1010/mL after the procedure. This is the first report of concentrating VHSV using an UF method that was specifically designed for seawater samples. In addition, the two-step UF procedure appears to be compatible with viral cell culture and qRT-PCR methods.
Keywords
Related Topics
Life Sciences
Immunology and Microbiology
Virology
Authors
Soo-Jin Kim, Jong-Oh Kim, Wi-Sik Kim, Myung-Joo Oh,