| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6133120 | Journal of Virological Methods | 2015 | 8 Pages |
Abstract
Isolation and serial propagation of the virus in MA-104 cells was confirmed by immunofluorescence assay, transmission electron microscopy, and polyacrylamide-gel electrophoresis of viral dsRNA. Eight (5.9%) of the electrophoretic profiles were characteristic of species B or D rotaviruses, which were not replicated in MA-104 cells.
Keywords
PBShuman colon carcinoma cell lineDEAEIFAhuman hepatocellular liver carcinoma cell linePAGERVACaco-2CPEDMEMDulbecco's modified Eagle Mediumimmunofluorescence assayCytopathic effectDiarrhoeapolyacrylamide gel electrophoresisTemTrypsinIsolationdiethylaminoethylRotavirusHepG2 cellsPhosphate buffered saline solutionTransmission electron microscopyUnitsCell culture
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Authors
Peter H. Otto, Jochen Reetz, Werner Eichhorn, Werner Herbst, Mandy C. Elschner,