Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6133169 | Journal of Virological Methods | 2015 | 6 Pages |
Abstract
Classical swine fever (CSF), caused by classical swine fever virus (CSFV), is a devastating disease of swine worldwide. Although a mandatory vaccination with the modified live vaccine C-strain has been implemented in China for decades, CSF remains a serious threat to the swine industry. To facilitate the control and eradication of CSF in China, the E2-based marker vaccine rAdV-SFV-E2, an adenovirus-delivered, alphavirus replicon-vectored vaccine, has been developed. Accordingly, an accompanying discriminatory test that allows differentiating infected from vaccinated animals (DIVA) is required. Here, the enhanced expression of Erns protein of CSFV was achieved in the methyltropic yeast Pichia pastoris by codon-optimization of the Erns gene, and an indirect enzyme-linked immunosorbent assay (iELISA) based on the yeast-expressed Erns (yErns) was developed and evaluated. The optimized iELISA was able to detect CSFV-specific antibodies in the serum samples from the CSFV-infected pigs as early as 6 days post-infection, and discriminate the CSFV-infected pigs from those vaccinated with rAdV-SFV-E2. The iELISA was evaluated using a panel of swine sera, and showed comparable sensitivity (94.6%) and specificity (97.1%), and the consistence rates with the virus neutralization test were 96.8% for CSFV-infected swine sera, 83.3% for C-strain-vaccinated swine sera, and 95.0% for field swine sera. In addition, the iELISA showed higher sensitivity (90.4%) compared with PrioCHECK CSFV Erns (59.6%). Taken together, the yErns-based iELISA is specific and sensitive, representing a promising DIVA test for E2-based marker vaccines against CSF.
Related Topics
Life Sciences
Immunology and Microbiology
Virology
Authors
Yuzi Luo, Lin Li, Sophia Austermann-Busch, Mei Dong, Jingjing Xu, Lina Shao, Jianlin Lei, Na Li, Wen-Rui He, Bibo Zhao, Su Li, Yongfeng Li, Lihong Liu, Paul Becher, Yuan Sun, Hua-Ji Qiu,