Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6133590 | Journal of Virological Methods | 2014 | 6 Pages |
Abstract
Encephalomyocarditis virus (EMCV) is one of the major zoonosis pathogens and can cause acute myocarditis in young pigs or reproductive failure in sows. In this study, a TaqMan-based real-time reverse transcription polymerase chain reaction (RT-PCR) assay targeting 3D gene of EMCV was developed and their sensitivities and specificities were investigated. The results indicated that the standard curve had a wide dynamic range (101-106 copies/μL) with a linear correlation (R2) of 0.996 between the cycle threshold (Ct) value and template concentration. The real-time RT-PCR assay is highly sensitive and able to detect 1.4 Ã 102 copies/μL of EMCV RNA, as no cross-reaction was observed with other viruses. These data suggested that the real-time RT-PCR assay developed in this study will be suitable for future surveillance and specific diagnosis of EMCV-infection.
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Authors
Wanzhe Yuan, Yingshuai Zheng, Mingtan Sun, Xiuyuan Zhang, Yan Qi, Jiguo Sun,