Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6133821 | Journal of Virological Methods | 2014 | 6 Pages |
Abstract
A 5Ⲡconjugated minor groove binder (MGB) probe real-time PCR assay was developed in this study for porcine sapelovirus (PSV) detection and quantitation. Two primers and a MGB probe for the 5Ⲡuntranslated region (UTR) gene were designed. The assay was capable of detecting about 103 copies/μl of standard template per reaction. Moreover, it does not detect any of the other RNA viruses that cause diarrhea disease in pigs. The coefficients of variation of intra- and inter-assay reproducibility were both lower than 2%. In 73 field fecal samples, PSV was detected in 46 samples using real-time PCR assay and only 32 samples with a conventional PCR assay. Therefore, the availability of this assay will facilitate further studies on the epidemiology of PSV infection and its role in swine disease.
Related Topics
Life Sciences
Immunology and Microbiology
Virology
Authors
Junwei Chen, Feng Chen, Qingfeng Zhou, Wei Li, Yanshan Chen, Yanhua Song, Xiangbin Zhang, Chunyi Xue, Yingzuo Bi, Yongchang Cao,