| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6133908 | Journal of Virological Methods | 2014 | 8 Pages |
Abstract
Characterizing the genomic sequences of influenza A viruses is important for pathophysiological and evolutionary studies. Noncoding regions (NCR) of influenza A virus have been shown to play critical roles in replication and transcription but their sequences are infrequently determined. In this study, a method employing poly(A) addition and SMART (switching mechanism at 5â² end of RNA transcript) technology is described for directly determining and discriminating both NCR ends of viral RNA (vRNA), complementary RNA (cRNA), or NCR and cap sequences from viral mRNA. This modified method may also be used to characterize the NCRs of influenza A virus samples in which the RNA has been degraded.
Keywords
Related Topics
Life Sciences
Immunology and Microbiology
Virology
Authors
Ruixue Wang, Yongli Xiao, Jeffery K. Taubenberger,