Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6133929 | Journal of Virological Methods | 2014 | 8 Pages |
Abstract
When real-time PCR was applied to environmental surveillance, the overall agreement rates between real-time PCR and the cell culture were 83.3% for all serotypes. Since real-time PCR has the advantages of rapid detection of viruses and minimum requirement of sampling volume as compared with ordinary cell culture, it is suitable to monitor oral poliovirus vaccine-like poliovirus in the environment, especially in areas where an oral vaccine is being replaced by an inactivated vaccine.
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Authors
Masae Iwai-Itamochi, Hiromu Yoshida, Mayumi Obara-Nagoya, Eiji Horimoto, Takeshi Kurata, Takenori Takizawa,