Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6133932 | Journal of Virological Methods | 2014 | 6 Pages |
Abstract
Fusion between the viral membrane of human immunodeficiency virus (HIV) and the host cell marks the end of the HIV entry process and the beginning of a series of post-entry events including uncoating, reverse transcription, integration, and viral gene expression. The efficiency of post-entry events can be modulated by cellular factors including viral restriction factors and can lead to several distinct outcomes: productive, latent, or abortive infection. Understanding host and viral proteins impacting post-entry event efficiency and viral outcome is critical for strategies to reduce HIV infectivity and to optimize transduction of HIV-based gene therapy vectors. Here, we report a combination reporter virus system measuring both membrane fusion and viral promoter-driven gene expression. This system enables precise determination of unstimulated primary CD4+ T cell subsets targeted by HIV, the efficiency of post-entry viral events, and viral outcome and is compatible with high-throughput screening and cell-sorting methods.
Keywords
Related Topics
Life Sciences
Immunology and Microbiology
Virology
Authors
Carisa A. Tilton, Caroline O. Tabler, Mark B. Lucera, Samantha L. Marek, Aiman A. Haqqani, John C. Tilton,