Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6134106 | Journal of Virological Methods | 2013 | 6 Pages |
Abstract
The reverse line blotting method is successful in genotyping common RVA strains in surveillance settings. For genotyping of rare strains, the number of probes on the blot can be expanded or a sequence-based method can be performed as a complementary approach on the samples that are positive in the detection PCR but negative in the reverse line blotting genotyping assay. The complete reverse line blotting protocol takes 8Â h to complete for 36 samples, with 3Â h hands-on-time. In conclusion, a RVA genotyping method that is accurate, cheap and fast is presented.
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Authors
Erwin Duizer, Bas van der Veer, Erwin de Bruin, Mark Zeller, Elisabeth Heylen, Marc van Ranst, Jelle Matthijnssens, Marion Koopmans, Harry Vennema,