Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6134200 | Journal of Virological Methods | 2013 | 5 Pages |
Abstract
18 monoclonal antibodies (MAbs) were isolated from 16 colonies, which were characterised by binding in ELISA and Western blotting, neutralisation of pseudotyped viruses and isotype. Specificity for the third variable (V3) region was detected in 5 of these antibodies, suggesting that the trimeric protein did not alter the main focus of the response compared with monomeric protein. 3 MAbs were identified as neutralising in a pseudotype assay, all of which were mapped to the V3 region. Although cross clade binding of antibodies was detected the neutralisation was C clade specific.
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Authors
Mark Hassall, Mark Page, Mark Robinson, Simon Jeffs, Ian Jones, Hongying Chen, Michael S. Seaman, Debbie Ferguson, Neil Almond,