| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6134221 | Journal of Virological Methods | 2013 | 8 Pages |
Abstract
Antibody-displaying phage library was selected after three rounds of panning against spring viraemia of carp virus (SVCV) by phage display technology. Eight positive clones which could produce soluble single-chain fragment variable (scFv) antibody induced by isopropyl-beta-d-thiogalactopyranoside (IPTG) were obtained. Dot blot results showed that the eight scFv antibodies could recognize SVCV. The soluble scFv antibodies showed a molecular weight 29Â kD by Western blot. All scFv antibodies could recognize SVCV proteins specifically without cross-reaction with other virus proteins by ELISA. Indirect immunofluorescence results showed that all of these scFv antibodies reacted positively with virus in the SVCV-infected cells. These scFv antibodies will be useful tools to establish immunological detection methods for SVCV.
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Authors
Hong Liu, Xiaocong Zheng, Feng Zhang, Li Yu, Xiaohua Zhang, Heping Dai, Qunyi Hua, Xiujie Shi, Wensheng Lan, Peng Jia, Li Yuan, Hong Liu,