Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6134361 | Journal of Virological Methods | 2013 | 4 Pages |
Abstract
The relative and analytical sensitivity of the APTIMA HPV test (AHPV, broad-spectrum, target amplification) and the PreTect HPV-Proofer (type-specific, target amplification) for the detection of HPV mRNA in various cell lines was compared. Equivalent relative sensitivity for the HPV 16-containing cell lines (2.5Â cells/ml with both CaSki and SiHa) was observed for the mRNA assays - and similar sensitivities were observed for the detection of HPV 18 (HeLa) and 45 (MS751); ranging from 2.5Â cells/ml (Proofer) to 25Â cells/ml (APTIMA). In relation to analytical sensitivity, again, the mRNA assays showed similar sensitivities to each other, ranging from 0.1 to 1Â cell per reaction for APTIMA and 0.1 to 10Â cells per reaction for PreTect HPV-Proofer (depending on cell line). Both mRNA assays consistently achieved a higher analytical sensitivity than a DNA based comparator - the Hybrid Capture 2 High-Risk HPV DNA test (hc2). This study indicates that mRNA tests had high analytical sensitivity, higher than a well established DNA-test based when using cell lines as target. Implications for clinical application are discussed.
Keywords
Related Topics
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Virology
Authors
Kate Cuschieri, Alison Hardie, Siri Hovland, Bente Hoaas, Frank Karlsen, Heather Cubie,