Diagnostic accuracy of a duplex real-time reverse transcription quantitative PCR assay for detection of African horse sickness virus

► Diagnostic sensitivity and specificity of the AHSV RT-qPCR assay were 97.8% and 99.9%, respectively. ► Diagnostic sensitivity and specificity of AHSV isolation were 44.2% and 99.99%, respectively. ► Utilisation of Standards for Reporting of Diagnostic Accuracy (STARD). ► Use of a 2-test 2-population Bayesian latent class model to estimate diagnostic accuracy without a reference “gold standard”.