| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6134386 | Journal of Virological Methods | 2013 | 7 Pages |
Abstract
⺠Botulinum toxin C-terminal heavy chain in a bi-cistronic baculovirus vector. ⺠One-step chromatographic purification was used to purify the recombinant protein. ⺠Enhanced green fluorescent protein was used to facilitate virus selection. ⺠Vaccination of the mice with recombinant protein induces protective immunogenicity.
Keywords
RhPVSf21eGFPBEVSAcMNPVIMACSNARESNAP-25BoNT/AAChAcetylcholineBaculovirusELISAEnzyme-linked immunosorbent assaylight chainheavy chaininternal ribosome entry sitebotulinum toxin Abaculovirus expression vector systemVaccineenhanced green fluorescent proteinImmobilized metal-ion affinity chromatographysoluble NSF attachment protein receptor
Related Topics
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Authors
Oliver B. Villaflores, Chein-Ming Hsei, Chao-Yi Teng, Ying-Ju Chen, Jiunn-Jye Wey, Pei-Yi Tsui, Rong-Hwa Shyu, Kuo-Lun Tung, Jui-Ming Yeh, Der-Jiang Chiao, Tzong-Yuan Wu,