Article ID Journal Published Year Pages File Type
6134418 Journal of Virological Methods 2013 8 Pages PDF
Abstract
► Bacterially expressed TriMV coat protein (CP) was purified as a soluble native form. ► Polyclonal antibodies produced against in vitro expressed TriMV CP in rabbits. ► Contrary to other viruses, TriMV antibodies reacted positively with native virions in DAS-ELISA. ► In DAS- and DAC-ELISA, antibodies specifically and sensitively detected TriMV in crude sap. ► Potential to develop high-throughput diagnostic methods for TriMV.
Related Topics
Life Sciences Immunology and Microbiology Virology
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