Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6134481 | Journal of Virological Methods | 2013 | 6 Pages |
Abstract
⺠A multiplex real-time PCR was developed for the detection and quantitation of Rotavirus A and Norovirus genogroup II. ⺠An internal extraction and amplification control was incorporated. ⺠Real-time PCR was compared to the current gold standard, enzyme immunoassay. ⺠Real-time PCR was significantly more sensitive than enzyme immunoassay. ⺠Quantitation demonstrated that the viral loads of both pathogens were ten times greater in stools children with diarrhea than in children without diarrhea.
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Authors
Tran Thi Ngoc Dung, Voong Vinh Phat, Tran Vu Thieu Nga, Phan Vu Tra My, Pham Thanh Duy, James I. Campbell, Cao Thu Thuy, Nguyen Van Minh Hoang, Pham Van Minh, Hoang Le Phuc, Pham Thi Ngoc Tuyet, Ha Vinh, Duong Thi Hue Kien, Huynh Le Anh Huy,