Article ID Journal Published Year Pages File Type
6134481 Journal of Virological Methods 2013 6 Pages PDF
Abstract
► A multiplex real-time PCR was developed for the detection and quantitation of Rotavirus A and Norovirus genogroup II. ► An internal extraction and amplification control was incorporated. ► Real-time PCR was compared to the current gold standard, enzyme immunoassay. ► Real-time PCR was significantly more sensitive than enzyme immunoassay. ► Quantitation demonstrated that the viral loads of both pathogens were ten times greater in stools children with diarrhea than in children without diarrhea.
Related Topics
Life Sciences Immunology and Microbiology Virology
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