| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6134539 | Journal of Virological Methods | 2012 | 10 Pages |
Abstract
⺠A novel denaturing heteroduplex tracking assay. ⺠The use of a single-stranded fluorescent probe based on the consensus V3 sequence of HIV-1 CCR5 tropic viruses. ⺠The use of Locked Nucleic Acid (LNA) “clamps” at both ends of heteroduplex DNA. ⺠The partially denaturing gel electrophoresis with formamide induced significant mobility shifts of heteroduplex DNA based on patterns of mutations. ⺠A useful research method for analysis of viral quasispecies and for genotypic prediction of HIV-1 tropism.
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Authors
Binshan Shi, Barbara Weiser, Linda M. Styer, Kimdar Kemal, Cheryl Brunner, Kathryn Anastos, Harold Burger,