| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6134540 | Journal of Virological Methods | 2012 | 6 Pages |
Abstract
⺠Quantitative single and dual target HIV-1 RT-PCR assays were developed. ⺠The technical performance of single/dual target assays in light of HIV-1 the epidemic was compared. ⺠Internal sample and external quantification controls were developed for robustness. ⺠The chosen assay proved to have good predictive value for monitoring HIV patients. ⺠This assay can substitute commercial assays when resources are limited.
Keywords
LTRLOQNIBSCRT-PCRPPVintCRF01_AEcycle thresholdpositive predictive valuenegative predictive valueribonucleic acidRNAIntegrasecoefficient variationLong terminal repeatLOD یا Limit of detectionReal time-polymerase chain reactionWorld Health OrganizationNational Institute for Biological Standards and ControlPDVlimit of detectionlimit of quantitationNPV یا negative predictive valueARTWHOUNAIDS
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Authors
Azzania Fibriani, Nadya Farah, Inri Kusumadewi, Suzan D. Pas, Reinout van Crevel, Andre van der Ven, Charles A.B. Boucher, Martin Schutten,