| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6134544 | Journal of Virological Methods | 2012 | 7 Pages |
Abstract
⺠A new test for dengue using one-step RT-PCR and one-enzyme RFLP is described. ⺠The test distinguishes between the 4 dengue serotypes, Yellow Fever, West Nile and SLE viruses. ⺠Results are obtained in 24 h with human sera or cultured virus and basic PCR and electrophoresis equipment. ⺠>95% specificity: serotype can be confirmed by sequencing and indirect immunofluorescence. ⺠The method identifies rapidly prevalent dengue serotypes; and assesses the risk of DHF/DSS in areas of prior exposure.
Keywords
DENVReverse-transcriptase PCRMVErestriction fragment lengthFlavivirusDHFRFLPRT-PCRDSSDengue hemorrhagic feverDiagnostic techniques and proceduresDengueDengue shock syndromeSLEpolymerase chain reactionMurray valley encephalitis virusSt. Louis encephalitis virusJapanese encephalitis virusYellow fever virusDengue virusWest Nile virusPanamaPolymorphismrestriction fragment length polymorphism
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Authors
Alma Ortiz, Zeuz Capitan, Yaxelis Mendoza, Julio Cisneros, Brechla Moreno, Yamitzel Zaldivar, Mariana Garcia, Rebecca E. Smith, Jorge Motta, Juan Miguel Pascale,