Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6134548 | Journal of Virological Methods | 2012 | 7 Pages |
Abstract
⺠A real-time RT-qPCR for enterovirus (EV) in cerebrospinal fluid was developed. ⺠An RNA chimera is used as internal control to monitor specimen process. ⺠Detection limit is 1875 copies/ml. ⺠The assay is reproducible and detects a large number of EV serotypes. ⺠The mean EV load in CSF specimens of patients with EV meningitis is 4.78 log10 copies/ml.
Keywords
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Virology
Authors
Romain Volle, Céline Nourrisson, Audrey Mirand, Christel Regagnon, Martine Chambon, Cécile Henquell, Jean-Luc Bailly, Hélène Peigue-Lafeuille, Christine Archimbaud,