Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6134552 | Journal of Virological Methods | 2012 | 4 Pages |
Abstract
Oyster mushroom spherical virus (OMSV) and oyster mushroom isometric virus (OMIV) are the causative agents of a fruiting body deformation disease in the edible mushroom Pleurotus ostreatus. The curing of these mycoviruses was facilitated by a serial transfer of infected mycelia onto a limited nutrient medium containing 1 mM of cAMP and 75 μg/ml of rifamycin (cAMP-rifamycin plate). The mycelia were grown on cAMP-rifamycin plates for 5 successive passages. ELISA and RT-PCR showed that the amount of mycoviruses inside the mycelia decreased significantly with increasing numbers of passages. The mycelia became free of viruses after 5 successive passages. Cultivation of the virus-cured mycelia on a mushroom compost medium produced a normal harvest, whereas the spawn infected with viruses failed to produce any fruiting bodies.
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Authors
Y.C. Kwon, D.W. Jeong, S.I. Gim, H.S. Ro, H.S. Lee,