Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6134563 | Journal of Virological Methods | 2013 | 6 Pages |
Abstract
⺠A RT-LAMP assay detecting rodent coronaviruses was developed. ⺠The detection sensitivity was 3.2-fold higher than that of RT-PCR. ⺠The detection sensitivity was 31.6-fold lower than that of nested RT-PCR. ⺠In a practical diagnostic test, the detection sensitivity of duplicate RT-LAMP with regard to the nested RT-PCR was 87.5%. ⺠In a practical diagnostic test, the detection specificity of duplicate RT-LAMP with regard to the nested RT-PCR was 100%.
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Authors
Ken-Ichi Hanaki, Fumio Ike, Rika Hatakeyama, Norio Hirano,