Article ID Journal Published Year Pages File Type
6134575 Journal of Virological Methods 2013 10 Pages PDF
Abstract
► The eukaryotic expression plasmids, pBudCE-NS4A and pBudCE-NS4B, were constructed. ► Two recombinant vaccinia viruses, rWR-NS4A and rWR-NS4B, were also constructed. ► The conditions of the priming-boosting strategy were established in the mouse model. ► The IFN-γ response of mice spleen cells was detected using ICS and ELISPOT assays. ► Three peptides of NS4A and NS4B protein were identified as H-2Kd restricted CTL epitopes.
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