| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6134647 | Journal of Virological Methods | 2013 | 4 Pages |
Abstract
⺠A FRET-based real-time PCR was developed for sensitive and specific detection of MCV. ⺠It allows reliable differentiation of the two MCV genetic lineages MCV1 and MCV2. ⺠The assay was validated on defined plasmid standards and clinical samples. ⺠The sensitivity of the assay was 3.3 viral copies/reaction; dynamic range was 10-109 viral copies/reaction. ⺠The novel assay is suitable for diagnostic application and epidemiological studies.
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Authors
Lea Hošnjak, Boštjan J. Kocjan, Blanka Kušar, Katja Seme, Mario Poljak,