| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6134668 | Journal of Virological Methods | 2013 | 7 Pages |
Abstract
⺠We investigated the performance of ViroSeq HIV-1 genotyping system v2.0 on strains identified in Senegalese patients. ⺠Amplification and sequencing rate were respectively 98% (147/150) and 96.6% (142/147). ⺠Primer B presented 100% success while the failure rate ranged from 1.4 to 71.4% for the other primers. ⺠No common polymorphic pattern or mutations between non-B subtypes that could predict the sequencing primer performances. ⺠Results suggest the need for an alternative technology to genotype non-B strains circulating in Senegal.
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Authors
Moussa Thiam, Halimatou Diop-Ndiaye, Khady Kebe, Nicole Vidal, Rokhaya Diakhate-Lô, Abou Abdallah Malick Diouara, Nafissatou Leye, Ousseynou Ndiaye, Amina Sow, Ndeye Fatou Ngom-Gueye, Souleymane Mboup, Coumba Toure-Kane,