| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6134749 | Journal of Virological Methods | 2012 | 6 Pages |
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed and optimized to detect bovine viral diarrhea viral (BVDV) RNA. The RT-LAMP assay is highly sensitive and able to detect 4.67Â ÃÂ 100Â copies of BVDV RNA. Additionally, the RT-LAMP method is capable of detecting both genotypes of BVDV. No cross-reaction with other bovine viruses was observed. The ability of RT-LAMP to detect BVDV RNA from bovine fecal swabs was also evaluated. Of the 88 fecal swabs, 38 were found to be positive by RT-LAMP assay, whereas 39 were positive by real-time RT-PCR. Taken together, the BVDV specific RT-LAMP method is highly specific and sensitive and can be used as a rapid and direct diagnostic assay for testing clinical samples.
⺠A set of primers specific to the BVDV genome was designed and used in the RT-LAMP. ⺠Both genotypes of BVDV can be detected by the RT-LAMP method. ⺠The RT-LAMP is a rapid, sensitive and specific diagnostic assay.
