Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6134818 | Journal of Virological Methods | 2012 | 5 Pages |
Abstract
⺠Reverse transcription triplex quantitative real time PCR assay for detection and quantitation of hepatitis E virus (HEV) RNA was optimised. ⺠The assay was able to detect at least 10 copies of the HEV genome/μl of isolated RNA and allows quantitation based on RNA standards. ⺠Application on biological samples revealed that amplification of two HEV loci increased the likelihood of HEV RNA detection. ⺠The use of RNA internal amplification control facilitated monitoring of false negative results.
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Authors
Petra Vasickova, Petr Kralik, Iva Slana, Ivo Pavlik,