Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6134840 | Journal of Virological Methods | 2011 | 15 Pages |
Abstract
⺠A microarray assay was developed for the detection of viruses infecting grapevine. ⺠cDNA was directly labelled using aminoallyl or 3D dendrimer methods and compared. ⺠Oligonucleotides between 27 and 75 base in length were evaluated and compared. ⺠Threshold levels were based on the ratio between viral specific and 18s rRNA controls. ⺠Results demonstrate the potential to detect plant RNA viruses without amplification.
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Authors
Ismail Abdullahi, Yana Gryshan, Michael Rott,