| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6134844 | Journal of Virological Methods | 2011 | 5 Pages |
Abstract
⺠Q-PCR assays may be of use to evaluate viral removal by filtration, instead of infectivity assays. ⺠However, it should be noted that the result obtained by Q-PCR might be underestimated for the removal of the manufacturing process when only degraded fragments of viral genome are targets for a Q-PCR assay. ⺠Hence the results of Q-PCR should be interpreted cautiously. ⺠Careful design of primers is needed to eliminate amplification by Q-PCR from viral DNA fragments.
Keywords
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Virology
Authors
Muneo Tsujikawa, Yuji Ohkubo, Maki Masuda, Hiroyuki Tanaka, Kadue Takahashi, Yuko Sasaki, Mikihiro Yunoki, Kazuyoshi Ikuta,