| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6134845 | Journal of Virological Methods | 2011 | 8 Pages |
Abstract
⺠Secretable NA (sNA) head domain of influenza A/Vietnam/DT-036/05 (H5N1) virus was expressed in mammalian cells. ⺠Cotransfection of a plasmid encoding nonstructural protein 1 (NS1) of influenza A/Puerto Rico/8/34 virus with the sNA plasmid increased sNA expression by 7.4 fold. ⺠The sNA/NS1 cotransfection protocol was optimized for rapid transient gene expression system in serum-free mammalian cell culture. ⺠The resulting sNA displayed comparable MW, glycosylation, Km for MUNANA, and Ki for oseltamivir carboxylate to those of H5N1 NA on the virus surface. ⺠This strategy may be applied for rapid expression of sNA of other strains of influenza virus as well as other recombinant proteins.
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Authors
Tarangsri Nivitchanyong, Suganya Yongkiettrakul, Jarin Kramyu, Supitcha Pannengpetch, Nanchaya Wanasen,